P-760 Effect of relaxin on human endometrial stromal cell characteristics

Abstract Study question Does relaxin (RLX) regulate decidualization and pro- antiangiogenic factors in primary human endometrial stromal cells (hESC), decidual (pD) hTERT-immortalized hESC (T hESC)? Summary answer RLX treatment enhances affects the mRNA expression levels of factors. What is known already Patients undergoing frozen embryo transfer an artificial cycle (AC-FET) are at a 2-fold increased risk for preeclampsia compared to natural cycle. This may be related absence corpus luteum FET cycle, which produces important hormones such as estrogen progesterone early pregnancy. These supplied during AC-FET, whereas other products luteum, RLX, not. Low maternal were found women who developed preeclampsia, but direct relationship between occurrence remains elucidated. design, size, duration Primary isolated from biopsies without pathology, pD side placenta uncomplicated pregnancies T purchased American Type Culture Collection. Cells decidualized with either 0.5 mM cyclic adenosine monophosphate (cAMP) or cocktail 10 nM estradiol, 1 µM cAMP (EPC) additionally treated 0, 0.3 ng/ml twelve days. Participants/materials, setting, methods RNA was on day zero all three cell types. level markers (prolactin (PRL), insulin like growth factor binding protein (IGFBP1)) well (vascular endothelial (VEGF); (PlGF); soluble Fms-like tyrosine kinase-1 (sFlt-1); endoglin (ENG)) evaluated by quantitative real time polymerase chain reaction (qRT-PCR), N = 3 biological replicates. Main results role chance Decidualization caused more noticeable changes morphology than EPC. While there no rise PRL (p 0.99) IGFBP1 0.95) group, after EPC showed significant increase both 0.0003) 0.0001). significantly (both p < 0.05, respectively), proangiogenic marker VEGF (P 0.05). A comparative analysis moderate effect PlGF. No detected levels. The ENG group both, 0.04) 0.01) control. dose dependent manner not hESC. 1ng/ml enhanced 0.002) PlGF 0.02) only pD. Limitations, reasons caution Our pilot study indicates that improves different models. wider approach needed investigate effects process Wider implications findings provide basis further studies elucidating low preeclampsia. could lead development improved prediction models new options assisted reproduction reduce e.g. AC-FET. Trial registration number Not applicable